Journal: bioRxiv

Article Title: UVB-Induced Genotoxic Stress Activates the DNA Damage Response and Innate Immune Pathways in Sea Urchin Coelomocytes

doi: 10.64898/2026.01.14.699502

Figure Lengend Snippet: (a) Viability of S. purpuratus coelomocytes and human peripheral blood mononuclear cells (PBMCs) measured 24 h after exposure to a range of UVB doses. For coelomocytes, mean values ± the standard deviation of biological triplicates (n = 3) are shown. For PBMCs, mean values ± the standard deviation of technical triplicates are shown. Individual points are overlaid as white circles. (b) Example of a comet with the head and tail region indicated. (c) Average tail DNA percent and (d) average percent of damaged cells (the percent of cells with any amount of DNA damage) in S. purpuratus coelomocytes challenged with 1,000 mJ/cm 2 UVB at 1, 6, and 24 h post-exposure (red bars) versus control, untreated coelomocytes (purple bars). Error bars are mean values ± the standard deviation of biological triplicates (n = 3). Individual points are overlaid as white circles. Significant differences between control and UVB-treated cells were analyzed using a one-way ANOVA and post-hoc TukeyHSD test (***, adjusted p value < 1 x 10 -3 ).

Article Snippet: In contrast, human peripheral blood mononuclear cells (PBMCs; ATCC PCS-800-011) demonstrated a sharp decline in viability following exposure to UVB with an LD 50 value of 871 mJ/cm 2 .

Techniques: Standard Deviation, Control

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Neutrophil-macrophage crosstalk via NETs–IL-17/VEGF/S100A9 axis promotes hepatocellular carcinoma progression

doi: 10.1186/s13046-025-03618-x

Figure Lengend Snippet: NETs facilitate M2d macrophage polarization upon co-culture with HCC cells. A Schematic diagram of the experimental procedure: M0 macrophages derived from THP-1 cells or human PBMCs, with or without NETs treatment, were co-cultured with normal hepatocytes (THLE-2) or HCC cells (HuH-7) for 24, 48, or 72 h. Cells were then harvested and macrophage polarization was analyzed by FCM. B FCM analysis of CD68 + CD86 + M1 and CD68 + CD206 + M2 macrophages polarized from THP-1-M0 according to procedure A. C , D Quantification of the fold change in the proportion of M1 (C) and M2 (D) polarized from THP-1-M0 from three independent experiments shown in B. E IF analysis of CD80 + M1 and CD206 + M2 polarized from THP-1-M0 after 48 h according to procedure A. F qPCR analysis of mRNA levels of M2d-related genes ( VEGF , IL-10 , TGF-β ) and M1-related genes ( iNOS , TNF-α ) in THP-1-M0 from the above co-culture systems of A for 48 h. G ELISA detection of the levels of M2d-related cytokines (VEGF, IL-10, TGF-β) and M1-related cytokines (iNOS and TNF-α) in the culture supernatant from the above co-culture systems of A (THP-1-M0). H Western blot analysis of VEGF and IL-10 expression in THP-1-M0 from the above A co-culture systems for 48 h. Protein levels were quantified by densitometric analysis and normalized to β-actin. Data are presented as fold change compared to the control group (right panel) White scale bars: 20 μm. Data are presented as mean ± SD. Ns, not significant. * p < 0.05, ** p < 0.01, *** p < 0.001. All data were analyzed using one-way ANOVA followed by the Newman-Keuls multiple comparison test

Article Snippet: Human peripheral blood mononuclear cells (PBMCs) were freshly isolated from healthy donors via Ficoll density gradient centrifugation (P9010, Solarbio, China).

Techniques: Co-Culture Assay, Derivative Assay, Cell Culture, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Control, Comparison